Journal: Nature Communications
Article Title: Inflammatory neuropathy in mouse and primate models of colorectal cancer
doi: 10.1038/s41467-026-76683-1
Figure Lengend Snippet: a Timeline of tumor cell injection in MaFIA mice and peripheral macrophage ablation using AP20187. b , c A statistically borderline reduction in DRG macrophage density in MC38 mice 24 h after ablation (F (2,8) = 3.686, p = 0.059). Two-sided unpaired t-test. Error bars = SEM. d , e A significant reduction in sciatic nerve macrophage density in MC38 mice 24 h after ablation (F 2, 6 = 17.5, p = 0.003; one-way ANOVA, Šidák). Error bars = SEM. f Worsened beam walk performance in MC38 mice post-ablation ( n = 8 per group, F 2, 26 = 4.253, p = 0.042, aligned rank transformed one-way ANOVA, Šidák’s multiple comparisons test). g Schematic of MC38 mouse serum collection and treatment of cultured bone marrow-derived macrophages (BMDMs). h , i Gene Ontology analysis and heatmaps reveal significant expression changes in BMDMs exposed to MC38 mouse serum, most notably, upregulation of pathways consistent with interferon stimulation and immune cell chemotaxis, alongside downregulation of cholesterol biosynthesis. Benjamini-Hochberg FDR-adjusted p -values. Source data are provided as a file. a , g Created in BioRender. Shepherd, A. (2026) https://BioRender.com/6vbp08x .
Article Snippet: Mouse Bone Marrow Derived Macrophages (mBMDMs; iXCells Biotechnologies 10MU-030) were maintained in T75 flasks according to manufacturer’s recommendations.
Techniques: Injection, Transformation Assay, Cell Culture, Derivative Assay, Expressing, Chemotaxis Assay